In vitro demonstration of tumor-specific common antigens and embryonal antigens in murine fibrosarcomas induced by 7,12-dimethylbenz(a)anthracene.

were tested at the first, fifth, and ninth transplant generation with spleen lymphocytes from BALB/c mice that were immunized against syngeneic tumors, allogeneic tumors, or embryo cells. Individual antigens were detected on fibrosarcomas at the first and fifth transplant passage, whereas they were undetectable on the eight tumors tested at the ninth passage. Lymphocytes sensitized against a pool of five C3Hf fibrosarcomas induced by 7,12-dimethylbenz(a)ant tiracene revealed cross-reacting antigens in one, seven, and eight out of the eight BALB/c fibrosarcomas tested at the first, fifth, and ninth transplants, respectively. Similarly, lymphocytes sensitized against embryo cells were cytotoxic on two fibrosarcomas out of the six tested at either the first or fifth passage and on six out of the seven tumors tested at the ninth passage.